Differential centrifugation purification and electron microscope examination of avian encephalomyclitis virus 應(yīng)用差速離心法提純禽腦脊髓炎病毒及電鏡觀察
The culture supernatants of heat - shocked and control a20 cells were collected to isolate exosomes through a sequence of differential centrifugation steps . briefly , cells were removed by centrifugation for 5 min at 300x gmax 在透射電鏡下hs一exo形態(tài)與exo類(lèi)似,呈50一sonm的雙層膜囊泡樣結(jié)構(gòu),大小比較均一,結(jié)構(gòu)完整。
The enriched stable isotope tracer technique combined with neutron activation analysis and differential centrifugation was utilized to investigate the subcellular distribution of rare earth elements sm and yb in rat liver Sm和, sup 168 yb作為示蹤劑,結(jié)合差速離心分離和中子活化分析技術(shù)naa初步研究了稀土元素sm和yb在大鼠肝臟亞細(xì)胞組分中的分布。
百科解釋
Differential centrifugation is a common procedure in microbiology and cytology used to separate certain organelles from whole cells for further analysis of specific parts of cells. In the process, a tissue sample is first homogenised to break the cell membranes and mix up the cell contents.